FastQCFastQC Report
Sun 20 Nov 2022
CO_010-COM.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_010-COM.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174
Sequences flagged as poor quality0
Sequence length25-361
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG302.555366269165247No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG282.385008517887564No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG282.385008517887564No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG252.1294718909710393No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG191.6183986371379897No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC181.5332197614991483No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA181.5332197614991483No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC181.5332197614991483No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT171.4480408858603067No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG151.2776831345826234No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG141.192504258943782No Hit
GCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT141.192504258943782No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT121.0221465076660987No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT121.0221465076660987No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT110.9369676320272573No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT110.9369676320272573No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG110.9369676320272573No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG110.9369676320272573No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT100.8517887563884157No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA100.8517887563884157No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT100.8517887563884157No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG90.7666098807495741No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC90.7666098807495741No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT90.7666098807495741No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT90.7666098807495741No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT90.7666098807495741No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAG80.6814310051107325No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA80.6814310051107325No Hit
CGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT80.6814310051107325No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG80.6814310051107325No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT80.6814310051107325No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG80.6814310051107325No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA80.6814310051107325No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG80.6814310051107325No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG80.6814310051107325No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGG70.596252129471891No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGC70.596252129471891No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT70.596252129471891No Hit
CCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT70.596252129471891No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC70.596252129471891No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC60.5110732538330494No Hit
CGCTGCGAATGCTCGGTGCACTTGAAGATCTGGCCGCCATAGGCGCGCTC60.5110732538330494No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG60.5110732538330494No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT50.42589437819420783No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT50.42589437819420783No Hit
CTCGACGATACCTAACTGTCTCATGTCGGCAATGGTTCCGTTGTTGAGAT50.42589437819420783No Hit
CCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT50.42589437819420783No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT50.42589437819420783No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAATATTAGGGAGGCGGCCATGGAATTCGCG50.42589437819420783No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT40.34071550255536626No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAG40.34071550255536626No Hit
CCTAATGTTACACACTGACTAGAGACTAGTGGCAAT40.34071550255536626No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT40.34071550255536626No Hit
CGTGCGTGAAGGAGAAAACTTCGCTGACTTCGTATCTAGCTGCACTGGTG40.34071550255536626No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAG40.34071550255536626No Hit
GGATAAGACGACTCTCAGGCGCGCCATGGAGCCGTTCTTCACCACGAAAG40.34071550255536626No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG40.34071550255536626No Hit
GTGCGTTGACCATGTACTCGACCGCGTCGGCGATCAGTTCGCGGCTCGGCAG30.2555366269165247No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAG30.2555366269165247No Hit
TGTCTGATATAAGTATAGTTAATCCTGCTCTTTTTTGGTTTCCATTTGCA30.2555366269165247No Hit
CAGGTTGGCCTCTCTGGAGACACCGAGCTGTGTGCAATCCAGGCCAAATG30.2555366269165247No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT30.2555366269165247No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGC30.2555366269165247No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCA30.2555366269165247No Hit
CTGCACAGATTTTATAATGTAATAAGCAAGAATACATTATCAAACGAACA30.2555366269165247No Hit
AGATTGTATGACGGGTTAGAAGCAGAGAACGATCCGGCCAACCGATCTAA30.2555366269165247No Hit
AGTCAACTTGGTTGAATTGGGTGTGTACGTCTCTGATATCAGAGCGCACT30.2555366269165247No Hit
TCTGATAAACTGTTTTGACAACTGGTTACTTCCCTAAGACTGTTTATATT30.2555366269165247No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGAGGCGGCCATG30.2555366269165247No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGAGGCGGCCATGGAATTCGCGACC30.2555366269165247No Hit
CACAGGATGCCTCTTTTCGCATGACAGAGATGCCTAAGTTCATTATCCAC30.2555366269165247No Hit
GCATCAGGCTTGTTCTGTAATTCGTTGTAGTAAATCTTAATTTCAGAACT30.2555366269165247No Hit
CTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT30.2555366269165247No Hit
GACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAA30.2555366269165247No Hit
GCACCCTATGCCATTACTTTCAAATATAATAGTTAAGATGACAAAAAACA30.2555366269165247No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAG30.2555366269165247No Hit
GCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT30.2555366269165247No Hit
CCACAAGCACGCCCGCTGCGTCTGACCAAGGCCCTCACTACCCGACCCTT30.2555366269165247No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG30.2555366269165247No Hit
CCAGTCAAGCAAATGGTAATATTAAGGAACATTTAAATTAATGACGTCAT20.17035775127768313No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACC20.17035775127768313No Hit
GAAGGTGTTGCCAAAGGAAATTAACATTTGAGTCAGTGGTCTGGGAGAGG20.17035775127768313No Hit
AGTTTATACCATTTAACTGGCTTTTAGCCAAAATTGTCACCTACTTGCTG20.17035775127768313No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTCGACTAG20.17035775127768313No Hit
CGCATACTTAATAAAAATTTATACTCTACACCACCTTCGTTATTACTTCA20.17035775127768313No Hit
CCTATATGGGTGACAGAGAGAGATGCAGTCAAAATAAAGGAATGGAATGG20.17035775127768313No Hit
CAATATCTGTAGCATCAAATTTGGGGAGCAATGTAAGTTCAAGAGAAGTC20.17035775127768313No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG20.17035775127768313No Hit
TGGAATAATCCATGGACTCGAATGCAATCATCATCGAATGGAATCGAATG20.17035775127768313No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT20.17035775127768313No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCTGGAATTCGCGAC20.17035775127768313No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT20.17035775127768313No Hit
CTGTTCACTTGTCTCTTACATCTTTCTTGGTAAAATCGTAGTTCGTAGTA20.17035775127768313No Hit
CGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTGTAGTTAAGCTGGT20.17035775127768313No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGGCTAG20.17035775127768313No Hit
GAAATAGTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAA20.17035775127768313No Hit
GCCGCCTCCCTAATGTTACACACTGACTAG20.17035775127768313No Hit
TTATAATGTCATTCTCCTTACTACAGACCCGCCTGAAAGTAGACACATCA20.17035775127768313No Hit
GACCACAAACATGTAGGAAACACGTGCTGTTAAGACAGGTGTCTGGCTGG20.17035775127768313No Hit
CAGTACTTGACCAATCTATCAAGCTGTAGTAAAAATCGTCTGCTAATGAT20.17035775127768313No Hit
ACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAA20.17035775127768313No Hit
CAACATCGAGAATCTATTATGAACAAGTGGCTAGATCTTTGGCGAGACTT20.17035775127768313No Hit
ACCCAGAGCAATAAATTCACTTGTTCCTGTTATTTAGTGTTTATCATAGA20.17035775127768313No Hit
TATCAACAGTCCTCAGCTGGCCAACCTATAAATGCAAAGCCATCCCAAAC20.17035775127768313No Hit
GGTCTCGTCCGAGATGATCTTGCGCAGCGGCAGGACCTCGCGCTGGTAAT20.17035775127768313No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCC20.17035775127768313No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA20.17035775127768313No Hit
TGTCATGGTGTCGGAAGGAAGTACGCCAATGGGGTAAAGGGGTCAGACGG20.17035775127768313No Hit
GGTGGTGCATGCCTGTAATCCCAGCTACTCAAGAGGCTGAGGCAGGAGAA20.17035775127768313No Hit
ATCAAAGAGATCCACAATCCGTATACAGCACACAGATTTTGATTCCTTGC20.17035775127768313No Hit
TCTTCAACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA20.17035775127768313No Hit
CCCATAAACCTCCCGCAAGGCAGAGGGAATATCAGCAGGGAGGGTGAGGT20.17035775127768313No Hit
TCGCGAATTCCATGGCCGCCTCCCTATGTT20.17035775127768313No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCA20.17035775127768313No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC20.17035775127768313No Hit
TTGAGTGTGGTAAGACTTTCGCCAGTGCGAATAATGGCAGTTTCCAAATC20.17035775127768313No Hit
CTAGTCAGTGTGTACATTAGGAGGCGGCCATGGAAT20.17035775127768313No Hit
ACAAAACACCACCAGCAATGTGCAACAAGGAAACATCGAAAGTACCACCA20.17035775127768313No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC20.17035775127768313No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTGACATTAGGGAGGCGGCCATGGAATTCGC20.17035775127768313No Hit
CCCCTGTCGTCTTTGTGCAGCGTGACCCAGTAGTCGCGCATGCGTTCTTCGG20.17035775127768313No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAG20.17035775127768313No Hit
GGCCGCCGGTGATGGCGCCGTCGACGACGAGGTCGTGGCCGTTGATGAAG20.17035775127768313No Hit
GATAAGGTTGTTGAGGACCTGAAGCAGGTGCTGGGAAACAAGCCTGAAAA20.17035775127768313No Hit
GGTTAAAAAAACATGGGTAAAGTTATTTTAGTTACAGGTGTTTCCAGAGG20.17035775127768313No Hit
CCGAAGGTCTGCAGCAGACATTGGGCAGGCCAGGGATGTCCCTGAGACTC20.17035775127768313No Hit
GATGAAGTGTCAGTTTCTATTGGAACGTCAAAGTTTGAAGGATCAATTGC20.17035775127768313No Hit
GTCGCGAATTCCATGGCCACCGTCCGGCTGTCTAGATGAACTAACACCTT20.17035775127768313No Hit
ATACCAGCCCATTCCGCATCACTCTTAGCGTATACGTGTTGGGCGGGAGT20.17035775127768313No Hit
CACGTCGCACCTATATCTGCGTGTTGCCTGTATATATATATACATGAGAA20.17035775127768313No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACAA20.17035775127768313No Hit
AGCGGGAAAGCCATGTATCTCTTGTAATTAGTTTGATCCTGTTCTATACC20.17035775127768313No Hit
CGCCATCGCAACCTTCTTCAATAAAGTTTCTTACAGGGGCCACATTCAAAG20.17035775127768313No Hit
CCATTCAACTCCATTCGATGTTTCCATTCGAGTTCATTCTATTATTCCAT20.17035775127768313No Hit
GGATGACAAAGGTGCAGGGGAACCTGAGATGCTCATGGGTGGTTCCAGGG20.17035775127768313No Hit
AAGTGTGTGTGTGTTTGGGGTCTATCTAGAAATAAAGCTTTGTAATAAAT20.17035775127768313No Hit
GCACCCTATGCCATTACTTTCAAATATAATAGTTAAGATGACAAAAAAAC20.17035775127768313No Hit
GACGATGCTCTCGTCGTCGGGGTGGGCGTGCACCATCATGAGTCGTCTGT20.17035775127768313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)